Journal: Nanoscale Advances

Article Title: Targeted delivery of berberine via ROS-sensitive polymersomes enhances its hepatoprotective activity in CCl 4 -intoxicated mice

doi: 10.1039/d5na00706b

Figure Lengend Snippet: Western blot analysis of oxidative stress, apoptosis, and autophagy markers in the liver (A). Mice were treated with vehicle, polimersomes (PS), carbon tetrachloride (CCl 4 ), CCl 4 + berberine (BER), and CCl 4 + BER-PS. Semiquantification of the expression of 4-hydroxynonenal (4-HNE) (B), hem oxygenase-1 (HO-1) (C), cleaved caspase-3 (D), caspase-9 (E), microtubule-associated protein 1 light chain 3 beta (LC3B)-I/II (F), p62 (G), and p21 (H). Each value represents the mean ± SD for 6 mice. *** p < 0.001 CCl 4 compared to control; # p < 0.05, ## p < 0.01, ### p < 0.001 CCl 4 compared to BER + CCl 4 and BER-PS + CCl 4 ; $ p < 0.05, $$ p < 0.01, $$$ p < 0.001 BER + CCl 4 compared to BER-PS + CCl 4 .

Article Snippet: Primary antibodies to extracellular regulated kinase 1/2 (ERK1/2) (#4695), phosphorylated ERK1/2 ( p -ERK1/2 Thr202/Tyr204, #4370), c-Jun N-terminal kinase 1/2 (JNK1/2) (#9252), phosphorylated JNK1/2 ( p -JNK1/2 Thr183/Tyr185, #4668), p38 (#8690), phosphorylated p38 (p-p38 Thr180/Tyr182, #4511), cleaved caspase-3 (#9661), and microtubule-associated protein 1 light chain 3 beta (LC3B)-I/II (#2775) were from Cell Signaling Technologies (Beverly, MA, USA), and GAPDH (HRP-60004) was obtained from Proteintech, (Rosemont, IL, USA).

Techniques: Western Blot, Expressing, Control

Journal: Biology

Article Title: Uncovering the Regulatory Role of Proteins in EBSS-Induced Autophagy Using RNA-Seq Analysis

doi: 10.3390/biology14101373

Figure Lengend Snippet: Autophagy is induced in NRK cells by EBSS. ( A ) NRK cells were incubated with EBSS for 0, 0.5, 1 and 2 h. LC3B-II protein levels were analyzed by immunoblotting. ( B ) NRK cells were incubated with EBSS, and cell survival rate was measured by the cck-8 kit. ( C ) NRK-GFP-LC3 cells were treated with EBSS for 0, 0.5, 1 and 2 h and images were captured by confocal microscope. Scale bar: 5 μm. ( D ) The average number of GFP-LC3 puncta per cell was counted in ( C ) at 0, 0.5, 1 and 2 h, respectively. GFP-LC3 puncta were quantified in ≥30 cells. *, p < 0.05.

Article Snippet: Microtubule-associated protein 1 light chain 3 beta (LC3B) antibodies and β-actin (ACTB) antibodies were purchased from Proteintech Group (14600-1-AP and 18420-1-AP, Wuhan, China).

Techniques: Incubation, Western Blot, CCK-8 Assay, Microscopy